For sequences longer than 100 bp, a fixed value of 37 mmol -1cm -1 is used. The absorbance factor is the conversion factor used to calculate the concentration of oligonucleotides or single stranded DNA, using the measured absorbance at 260 nm (with a 1 cm lightpath). The molecular weight of ambiguous characters are calculated as the average of its constituents, for example R is calculated as (mwA + mwG) / 2. The SS (single-stranded) molecular weight is calculated for the phosphorylated, protonated form of the molecule. Refer to the Primer Design tutorial for formulas for these methods. The melting temperature of the double stranded molecule is calculated using the nearest-neighbour method for sequences less than 50 bp and the long probe method for sequences which are longer. Ambiguous characters are treated as fractions of bases, for example an R is counted as 0.5 A and 0.5 G. The pie chart represents the proportions of the four different bases (A, T, C and G for DNA, A, U, C and G for RNA). The various functions are outlined below. The data in the Calculator window apply to either the currently selected region of the sequence, or the whole sequence if nothing is selected. This section outlines how to use the tool, and how the various data is computed. The results are presented in an easy-to-read format. The Calculator Tool provides data on a variety of properties of either your protein or DNA sequences. Properties Calculator for DNA and Proteins Properties Calculator for DNA and Proteins Home |
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